We’ve covered cheap DIY PCR solutions a couple of times, but there’s always room for one more PCR device. PocketPCR from the wonderful GaudiShop is a $110 PCR kit. The project has a well thought out assembly detailed in this instruction manual, but it will require a tiny bit of soldering. The USB powered device […]
Tag: PCR
A qPCR device for $300
A real-time polymerase chain reaction (real-time PCR), or quantitative polymerase chain reaction (qPCR), is a modification to normal PCR where one monitors the amplification of a targeted DNA molecule during the PCR. We’ve written before about our favorite cheap solutions for PCR, but check out this interesting paper on building your own quantitative-PCR (qPCR) machine written by Geoffrey Mulberry, […]
Accurate Enzymatic Assays in PCR Tubes
To achieve reproducible findings in many enzymatic assays, the substrate and sample must be incubated at specific temperatures without evaporation. If you don’t want to pay thousands for a 96-well plate incubator, than there are two main ways to do this: -The first is preparing 1.5mL Eppendorf tubes and thoroughly vortexing the final solution to […]
Cheap Solutions to Thermocyclers for PCR
You’re going to spend how much on a thermocycler? That’s crazy! Why not get miniPCR, a beautiful PCR kit with some awesome software for just $650 or OpenPCR, a thermocycler kit for just $500. Not cheap enough? Go the full DIY and you can build on for less than $100. Do you just refuse to […]