miCube, not to be confused with µCube, is an a open and modular framework for the design and assembly of florescence microscopy optical devices, including devices with TIRF and widefield as well as an advertised confocal capabilities. The miCube has a peer-reviewed manuscript demonstrating it’s performance written by Koen Martens, Sam van Beljouw, Simon van […]
Tag: TIRF
A step-by-step guide to building a prism-type TIRF microscope
Total Internal Reflection Florescence (TIRF) Microscopy is a technique used to observe a very thin region of sample directly at the surface of an interface (coverslip) without obtaining background interference from the rest of the sample volume. Dalton Gibbs, Anisa Kaur, Anoja Megalathan, Kumar Sapkota, and Soma Dhakal have published a wonderful paper entitled “Build Your Own Microscope: Step-By-Step […]
liteTIRF – An Economic TIRF Microscope
We’ve covered a lot of confocal imaging systems on this blog, as those tend to be the real bank breakers, but have a look at this beautifully compact TIRF platform: the LiteTIRF. The website includes software, a thorough components list, and a neat little CAD drawing to show you how everything goes together. The components […]