Fluorescence-lifetime imaging microscopy (FLIM) is a microscopy technique used to measure the lifetime of a fluorophore rather than its intensity. Sensitive to the sample region’s micro-environment, lifetime measurements aren’t limited to measuring molecule concentration like traditional microscopy, but can also indicate environmental effects such as pH or viscosity. Additionally, this technique is not as affected […]
Tag: two-photon
Easy Expansion of Field of View in Two-Photon Set Up
Two-photon micrsocopy is useful for reducing photo-damage and seeing deep into tissue samples. First patented in 1990 by Winfried Denk and James Strickler, it’s a technique that has been used for decades in neuroscience as a minimally invasive way to image neurons in real time. But we’ve already covered how to make one of those […]
Ytterbium laser for $13,000.
One of the largest obstacles to two-photon DIY microscopy is the acquisition of a pulsed laser system, which generally hits the $100,000 range. Evan Perillo, Justin McCracken, Daniel Fernée, John Goldak, Flor Medina, David Miller, Hsin-Chih Yeh, and Andrew Dunn have a 2016 paper detailing the construction and characterization of a Ytterbium laser at 1060 […]